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In vitro studies on liposomal amphotericin B obtained by supercritical carbon dioxide-mediated process.

Kadimi US, Balasubramanian DR, Ganni UR, Balaraman M, Govindarajulu V

Food Engineering Centre, Central Food Technological Research Institute, Mysore, India. udaya@cftri.res.in

Nanotechnology in drug delivery is a rapidly expanding field. Nanosized liposomal preparations are already in use for efficient drug delivery with better therapeutic indices. Existing methods of liposome preparation are limited by problems of scale-up, difficulty in controlling size, and intercalation efficiency. Here we prepare amphotericin B-intercalated liposomes by a novel process where amphotericin B and purified phosphatidyl choline are solubilized in suitable solvent and precipitated in supercritical fluid carbon dioxide (known as a gas antisolvent technique), to obtain microsized particles that are subsequently introduced into a buffer solution. The morphology of liposomes was characterized through a phase-contrast microscope, and the particle size distribution studied by laser technique showed nanosize with a narrow range of size distribution (between 0.5 and 15 microm) and a higher intercalation efficiency. In vitro studies conducted using Aspergillus fumigatus (MTCC 870) strain proved to be efficient in the retardation of the growth of the organism.

Published 10 December 2007 in Nanomedicine, 3(4): 273-80.
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